Facilities | Hoshino Laboratory Animals,Inc.

Mice	HR−１
	HRM-2
	NSY-A^y

Rats	ZFDM
	OLETF & LETO

	Animal production facilities

【 Animal husbandry 】

Temperature	21-27℃
Humidity	50-80%
Lighting	300 lx　【Light】 7:00-19:00 【Dark】 19:00-7:00
Air	Sterilized by 3-stage filters, front, middle, and final (HEPA)
Aeration	Pan-type air conditioner-diffuser blowout style

【 Feeds 】
Autoclaved solid pellets from Nihon Nosan Kogyo K.K. are used.

【 Production style 】
　① Closed colonies 　Maintained and produced by cyclic mating system.
　② Inbred strains 　Foundation stock colonies: The "seed stock" of each strain is maintained and produced by brother-sister mating. 　Multiplication colonies: Animals from stock colonies are multiplied by brother-sister mating to produce production colony animals and F1 production animals. 　Production colonies: These colonies consist of animals from multiplication colonies and they are multiplied by non-brother-sister mating to produce laboratory animals to be used for experiments.

【 Entry 】
　Anyone entering a barriers-system animal room must remove clothes, sterilize his/her hands and feet, take a shower, and then wear sterilized work clothes, a cap, a mask, and rubber boots before entering.